masoud keikha; hossein ali rahdar; Shahram Shahraki- Zahedani
Volume 25, Issue 5 , November and December 2018, , Pages 679-686
Abstract
Background and Aim: Non-tuberculosis mycobacteria is acid-fast bacteria that lives in environmental sources such as: water, soil, dust, milk and decaying vegetables. Based on Runyon’s classification these bacteria classified in tow group of slow and rapid growing mycobacteria that both of them ...
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Background and Aim: Non-tuberculosis mycobacteria is acid-fast bacteria that lives in environmental sources such as: water, soil, dust, milk and decaying vegetables. Based on Runyon’s classification these bacteria classified in tow group of slow and rapid growing mycobacteria that both of them isolated from clinical specimens.The purpose of this study is evaluation of three housekeeping genes in identification and differentiation of non-tuberculosis mycobacteria.Methods: In our study we obtained 16S rRNA, rpoB and hsp65 sequences of twenty two slow and rapid growing mycobacteria from Genebank (www.ncbi.nlm.nih.gov). Then this sequences aligned and transferred to MEGA 5.0 program. Finally phylogenetic relationships were determined by constructing 16S rRNA, rpoB and hsp65 genes tree using the neighbor-joining method with Kimura 2-Parameter model.Results and conclusion: Phylogenetic analysis based on 16S rRNA, rpoB and hsp65 gene sequences indicated that except of rpoB gene, other genes cannot identificated and separate of some species. Also we found that for identification both of rapid growth mycobacteria (RGM) and slow growth mycobacteria (SGM)rpoB gene is the best option.due to findings of this study, it seems that for appropriate and accurate identification of non-tuberculosis mycobacteria we must studied rpoB and hsp65 genes simultaneously
Microbiology
Behnam Hashemi; saeid taghiloo; Esmaeil allahmoradi; Morteza Karami; Hossein ali Rahdar
Volume 25, Issue 3 , July and August 2018, , Pages 303-308
Abstract
AbstractIntroduction: Portulaca oleracea is one of the most utilized herbs in the world. According to some antibacterial effect of plants extracts, it seems that this extracts can be used in the bacterial infection treatments. In order to assessment of antibacterial effect of the Portulaca oleracea extract, ...
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AbstractIntroduction: Portulaca oleracea is one of the most utilized herbs in the world. According to some antibacterial effect of plants extracts, it seems that this extracts can be used in the bacterial infection treatments. In order to assessment of antibacterial effect of the Portulaca oleracea extract, we determined minimum inhibitory and minimum bactericidal concentration of the Portulaca oleracea extract on the pathogenic bacterial species that antibiotic resistant pattern was determined in this species.Methods: Hydro-Alcoholic extract of the Portulaca oleracea was harvested. Bacterial species including S. aureus, P. aeruginosa, L. monocytogenes, A. baumannii, E. coli and K. pneumonia was collected from the Iran pasteur institute. Antibiogram was determined by disk diffusion method and then MIC and MBC of Hydro-Alcoholic extract of the Portulaca oleracea was determined.Results: Highest MIC concentration was observed in P. aeruginosa, L. monocytogenes, E. coli and K. pneumonia. While highest MBC concentration related to L. monocytogenes.Conclusion: Herbal antibacterial extracts can be useful in human infection control. In order to this goal, in this study antibacterial effect of Portulaca oleracea extract determined against six important pathogens. According to Our results hydro-alcoholic extract of Portulaca oleracea show antibacterial effect on human pathogens including E. coli, K. pneumonia, S. aureus, A. baumannii, P. aeruginosa and L. monocytogenes.